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Calcium Signaling Protocols

'Methods in Molecular Biology'. Auflage 2005. Sprache: Englisch.
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In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 i... weiterlesen


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Calcium Signaling Protocols als Buch
Titel: Calcium Signaling Protocols

ISBN: 1588294420
EAN: 9781588294425
'Methods in Molecular Biology'.
Auflage 2005.
Sprache: Englisch.
Herausgegeben von David G. Lambert

August 2005 - gebunden - 359 Seiten


In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. " This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.


Part I. General

Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations
Alec W. M. Simpson

Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert

Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
Adriaan Nelemans

Part II. Specialist Measurement Systems

Confocal Microscopy: Theory and Applications for Cellular Signaling
Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars

Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
Anthony J. Morgan and Andrew P. Thomas

Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer
Ian C. B. Marshall, Izzy Boyfield, and Shaun McNulty

Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Ian C. B. Marshall, Davina E. Owen, and Shaun McNulty

Part III. Measurement of Ca2+ Channel Activity

Measurement of Ca2+ Entry Using 45Ca2+
Mercedes Villarroya, Manuela G. López, María F. Cano-Abad, and Antonio G. García

Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding
Kazuyoshi Hirota and David G. Lambert

Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons
Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani

Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores

Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells
Alison Skippen, Philip Swigart, and Shamshad Cockcroft

Measurement of Inositol(1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
Darren Smart

Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay
Robert A. Wilcox

Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann

Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
Aldebaran M. Hofer

Part V. Specialist Measurement Techniques

Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
Hideo Kanaide

Measurement of Calcium and Movement in Heart Cells
Leong L. Ng and Paulene A. Quinn

Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch

Measurement of Cytosolic-Free Ca2+ in Plant Tissue
Martin R. McAinsh and Carl K.-Y. Ng

Part VI. Ca2+-Sensitive Targets

Assay and Purification of Calmodulin-Dependent Protein Kinase
Rajendra K. Sharma

Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
Rajendra K. Sharma

Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Danuta Kosk-Kosicka



From Reviews of the First Edition...
"...will find a place on the bench in any lab that is moving into calcium signaling, or expanding its repertoire of techniques in calcium signaling. It will be valuable as a resource for...years to come." -Cell Biology International
"This book achieves much...For anyone remotely interested in Ca2+ it will be a very valuable resource in their lab" -Quarterly Review of Biology
". . .recommended to investigators interested in calcium measurement using optical methods. "-Doody's Health Sciences Book Review Journal
"Most protocols identify required materials such as buffers, reagents and cell lines. Methods are presented in a step-by-step format. Hopefully these protocols are adaptable by scientists for use in specific research. Certainly, the methods are specific enough not to leave the researcher stranded. Calcium Signaling Protocols is recommended for academic health and science libraries who support academic research."-E-Streams

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