Cardiovascular Development

Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method. - Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample. - RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells. - Preparation of Neonatal Rat Papillary Muscles for Contractile Studies. - In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine. - Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells. - Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining. - Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue. - Laser-induced Choroidal Neovascularization in Rats. - Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain. - Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy. - Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues. - Visualization of Fundus Blood Vessels. - Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements. - Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery. - Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus. - Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.