ATM Kinase

Assaying Radiosensitivity of Ataxia-Telangiectasia. - Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology. - ATM Gene Mutation Detection Techniques and Functional Analysis. - An HTRF® Assay for the Protein Kinase ATM. - ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using Cellomics
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ArrayScan VTI Platform. - Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci. - Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy. - Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging. - Zn(II) Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein. - Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry. - Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS). - Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies. - Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays. - ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry. - Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling. - Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation. - Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics. - ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks. - Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System. - Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks. - Live Cell Imaging to Study Real-Time ATM-Mediated Recruitment of DNA Repair Complexes to Sites of Ionizing Radiation-Induced DNA Damage. - Analyzing Heterochromatic DNA Double Strand Break (DSB) Repair in Response to Ionizing Radiation. - Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair. - Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts. - Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage. - Study of ATM Phosphorylation by Cdk5 in Neuronal Cells. - DNA Damage Response in Human Stem Cells and Neural Descendants. - A Patient-Specific Stem Cell Model to Investigate the Neurological Phenotype Observed in Ataxia-Telangiectasia. - Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells. - Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures.