This volume details the phenotypic characterization of Staphylococcus aureus, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Staphylococcus aureus: Methods and Protocols is a valuable resource for anyone interested in this fascinating and developing field.
Inhaltsverzeichnis
Measurement of Staphylococcus aureus Pigmentation by Methanol Extraction. - Analysis of Murein Hydrolases and Proteases through Zymography. - Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants. - Quantitative Hemolysis Assays. - In Vitro Assay for Quantifying Clumping of Staphylococcus aureus. - Measuring Staphylococcal Promoter Activities using a Codon-Optimized ß-galactosidase Reporter. - Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays. - Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity. - Quantification of Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy. - Growth of Staphylococcus aureus using a Rotary Cell Culture System. - Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus. - Quantifying Staphylococcus aureus Membrane Potentialusing Flow Cytometry. - Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples. - A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins. - An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood. - Bone Marrow-Derived Macrophage Infection Assay. - Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins. - Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm.
