DNA Topoisomerases

DNA topoisomerases and gyrases have been studied thoroughly from a biochemical perspective, and expanding research at the molecular and cellular level shows great promise. This book gives a compendium of protocols that extends into such new areas of research.

1. Introduction: Emerging Themes in DNA Topoisomerase Research. - 2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis. - 3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers. - 4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II. - 5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA. - 6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer. - 7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases. - 8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase III? . - 9. ChIP-on-Chip Analysis of DNA Topoisomerases. - 10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation. - 11. Binding of DNA Topoisomerases I and II to Replication Origins. - 12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors. - 13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells. - 14. Assaying Topoisomerase II Checkpoints in Yeast. - 15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction. - 16. Top2 SUMO Conjugation in Yeast Cell Lysates. - 17. Analysis of SUMOylation of Topoisomerase II? with Xenopus Egg Extracts. - 18. The Dynamics of DNA Topoisomerase II? in Living Cells. - 19. Depletion and Mutation of Topoisomerase II in Animal Cells.