Metabolic Gene Clusters

This volume provides a comprehensive guide of cutting-edge technologies applied in the discovery of metabolic gene clusters across different domains of life. Chapter guide readers through cluster identification, characterization, evolutionary analyses, synthetic assembly, pathway re-constitution, cluster regulation and activation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Metabolic Gene Clusters: Methods and Protocols aims to spur new discoveries in the field of metabolic gene clusters and bring different areas of research closer together.

Generalized Gene Cluster Detection Using CLOCI. - In Silico Identification and Comparative Synteny of Biosynthetic Gene Clusters in Plants. - Mining Metabolic Diversities Across Genomic Neighborhoods in Plants. - Identification of Stress-induced Biosynthetic Gene Clusters in Plants. - Investigating Intraspecific Diversity of Metabolic Gene Clusters with MLPA and Synthetic Probes. - Synteny Analysis and Visualization of Gene Clusters. - Automating Plasmid Construction for High-throughput Heterologous Expression of Fungal Gene Clusters. - Molecular Lego in the Green Factory: Engineering Plant Metabolism with Golden Gate Assembly. - Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae. - Characterization of Bioactive Saponins from Sea Cucumbers. - Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes. - Cut& Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana. - Mapping the 3D Chromosome Organization of Streptomyces by Hi-C. - Mapping the 3D Chromosome Organisation of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).