Fusobacterium nucleatum

This volume presents a collection of both classic and modern experimental methods designed to highlight the biology, pathogenicity, and host interactions of Fusobacterium nucleatum. The chapters in this book provide practical guidance spanning microbiology, molecular biology, imaging, genetic engineering, metabolomics, and in vivo modeling. Some of the topics discussed in this volume are methods for visualizing and isolating F. nucleatum within its native oral environment; essential cellular processes such as surface protein assembly and peptidoglycan biosynthesis; protocols for asymmetric coculture systems; 3D tumor spheroid models; untargeted metabolite profiling of infected mammalian cells; and several in vivo platforms from murine models of periodontitis, oral cancer, and CRC to a Caenorhabditis elegans infection model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Fusobacterium nucleatum: Methods and Protocols is a fundamental resource for both new and experienced researchers looking to investigate F. nucleatum virulence mechanisms, colonization strategies, and its increasing contributions to diseases such as CRC, oral squamous cell carcinoma, and periodontitis.

CLASI-FISH Imaging of
Fusobacterium
in Oral Microbial Communities. - Isolation and Characterization of
Fusobacterium nucleatum
Subspecies from Oral Clinical Specimens. - A Droplet Digital PCR Approach for the Quantitative Detection of
Fusobacterium nucleatum
in Formaldehyde-Fixed Paraffin Embedded Tumor Tissue. - Enhanced Transformation Efficiency in
Fusobacterium nucleatum
by Bypassing Restriction Modification Systems: A Methylation Guided Protocol. - A Conditional Plasmid System for Markerless Gene Deletion in Genetically Recalcitrant
Fusobacterium nucleatum
subsp.
Animalis. -
Gene Silencing by CRISPR Interference in
Fusobacterium nucleatum. -
Methods for the Evasion of Restriction-Modification Systems During Genetic Engineering of
Fusobacterium
Species. - Tracking Surface Protein Assembly and Peptidoglycan Biosynthesis in
Fusobacterium nucleatum. -
Isolation of
Fusobacterium nucleatum
-Induced Host Exosomal sRNAs. - Isolation of Primary RNA for Differential RNA Sequencing in
Fusobacterium nucleatum. -
Isolation and Visualization
Fusobacterium nucleatum
Microcompartments. - Isolation and Proteomic Characterization of Outer Membrane Vesicles in
Fusobacterium nucleatum. -
An Asymmetric Gas Coculture System for Oral Pathobionts and Gingival Epithelial Cells. - A 3D Tumor Spheroid Platform to Model
Fusobacterium nucleatum
Induced Tumorigenesis. - Fluorescence and Electron Microscopy of
Fusobacterium nucleatum
-Induced Autophagy. - Mass Spectrometry-Based Untargeted Metabolite Profiling to Discover Molecular Consequences of Mammalian Cell Infection with
Fusobacterium nucleatum. -
A Mouse Model for
Fusobacterium nucleatum
-Mediated Experimental Periodontitis. - A Murine Floor-of-Mouth Model for Investigation of
Fusobacterium nucleatum
-Associated Oral Squamous Cell Carcinoma. - Modeling
Fusobacterium nucleatum
-Driven Colorectal Cancer in
Apc
-Mutant Mice. -
Caenorhabditis elegans
as a Model Host for
Fusobacterium nucleatum.