Immunoproteomics

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Introduction to the Immune System. - Immunoproteomics: Current Technology and Applications. - Antigen Identification Using SEREX. - Antigen Discovery Using Whole-Genome Phage Display Libraries. - Methods and Applications of Serological Proteome Analysis. - Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins. - Identification of the Antigen Content of Electroimmunoprecipitates. - An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus. - In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections. - Chemo-Enzymatic Production of
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Glycopeptides for the Detection of Serum Glycopeptide Antibodies. - Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures. - Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization. - Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes. - Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis. - Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification. - Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology. - Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis. - Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry. - Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes. - Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes. - Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics. - Commercial Considerations for Immunoproteomics.