DNA Damage Detection

This volume explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and timely, DNA Damage Detection: Methods and Protocols serves as an ideal guide for readers who are unfamiliar with DNA damage as well as experts seeking protocols to adapt to their own research.

Modified Alkaline Comet Assay to Detect Oxidative DNA Damage. - Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis. - Quantitative Measurement of 8-Hydroxy-2 -Deoxyguanosine in Serum/Plasma and Urine Using an Enzyme-Linked Immunosorbent Assay. - Radiation-Induced Gamma-H2AX Foci Staining and Analysis. - Non-Radiolabeled Radioresistant DNA Synthesis and S-Phase Checkpoint Analysis. - Constant Field Gel Electrophoresis Analysis for DNA Double-Strand Break and Its Repair. - Detection of Chromatid Break and Micronucleus Formation Induced by Low- and High-LET Irradiation. - Measurement of DNA Polymerase and Microhomology-Mediated End-Joining Activities by Gel Electrophoresis and smFRET Techniques. - UV-Induced DNA Damage Detection by ELISA Analysis. - G2 Premature Chromosome Condensation (G2-PCC) by Calyculin A to Analyze DNA Double-Strand Breaks and Their Repair. - Mitotic Shake-Off and Cell Cycle Synchronization. - DCFDA Assay for Oxidative Stress Measurement in Fluorometer. - HPRT Mutation Assay for Chinese Hamster Ovary Cells. - Detection of De Novo Mutations by Sequencing Reduced Representation Libraries. - Chemical-Induced DNA Damage Identification by High-Throughput Detection of DNA Double-Strand Break. - Assessing DNA Damage Through the Cell Cycle Using Flow Cytometry. - A Mass Spectrometry-Based Proteomics Workflow for Concurrent Profiling of Protein Thiol Oxidation and Phosphorylation. - Analyzing Homologous Recombination Using Antibiotic Marker Substrates in Mammalian Cells.