Preparative Chromatography Techniques

This book describes modern preparative chromatography techniques and their application in the separation of natural products, synthetic intermediates, metabolites, macromolecules, and biologically active substances, e. g. new lead compounds from microorganisms and both marine and terrestrial higher organisms. The authors focus their book on the applications and aim to provide the reader with a rapid idea of how to obtain milligram or gram quantities of pure constituents from complex mixtures with minimum effort.
Among others different pressure LC methods, including preparative HPLC and medium-pressure LC are treated and new all-liquid techniques, e. g. centrifugal partition chromatography, are discussed in detail. Descriptions of apparatus and operation procedures together with numerous examples of actual separations - often widely scattered in literature - are provided. This completely revised second edition has been enlarged by chapters on macromolecule and enantiomer separation and on preparative GC.

1 Introduction. - 2 Sample Preparation and Purification. - 3 Planar Chromatography. - 4 Special Column Chromatography. - 5 Preparative Pressure Liquid Chromatography. - 6 Preparative Gas Chromatography. - 7 Countercurrent Chromatography. - 8 Separation of Macromolecules. - 9 Separation of Chiral Molecules. - 10 Separation Strategy and Combination of Methods. - 11 Subject Index.