Microbial Proteomics

Filter-Aided Sample Preparation for Proteome Analysis. - Protein Enrichment from Highly Dilute Samples with StrataClean. - Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins. - Enrichment of Cell Surface-Associated Proteins in

Gram
⁺
Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis. - Preparation of Bacterial Magnetosomes for Proteome Analysis. - Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics. - Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS. - Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms. - Proteomic Signatures in
Staphylococcus aureus. -
How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in
Bacillus subtilis. -
Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE. - Sulfur-
³⁴
S and
³⁶
S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses. - Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (
¹⁵
N). - Next-Generation Trapping of Protease Substrates by Label-Free Proteomics. - In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings. - Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography. - Phosphoproteomics in Microbiology: Protocols for Studying
Streptomyces coelicolor
Differentiation. - Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria. - Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions. - Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies. - Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter. - Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses.