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Microfluidics Diagnostics

Methods and Protocols

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This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.

Inhaltsverzeichnis

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids. - DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology. - On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples. - Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device. - Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs). - Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows. - Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis. - Microfluidic Cartridge for Bead-Based Affinity Assays. - A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level. - Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets. - A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption. - An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics. - Microfluidic 3D Cytotoxic Assay. - Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach. - Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

Produktdetails

Erscheinungsdatum
17. Mai 2024
Sprache
englisch
Seitenanzahl
268
Reihe
Methods in Molecular Biology
Herausgegeben von
Valérie Taly, Stéphanie Descroix, Karla Perez-Toralla
Illustrationen
XII, 255 p. 74 illus., 70 illus. in color. With online files/update.
Verlag/Hersteller
Produktart
gebunden
Abbildungen
XII, 255 p. 74 illus., 70 illus. in color. With online files/update.
Gewicht
705 g
Größe (L/B/H)
260/183/21 mm
Sonstiges
Book w. online files / update
ISBN
9781071638491

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