Plant Cold Acclimation

Measuring Freezing Tolerance: Survival and Regrowth Assays. - Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays. - Conducting Field Trials for Freezing Tolerance Breeding in Cereals. - A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance. - Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation. - Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions. - Identification of Arabidopsis Mutants with Altered Freezing Tolerance. - Infrared Thermal Analysis of Plant Freezing Processes. - Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues. - Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images. - Proteomic Approaches to Identify Cold Regulated Soluble Proteins. - Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins. - Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry. - A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition. - Quantification of Superoxide and Hydrogen Peroxide in Leaves. - Estimating Ice Encasement Tolerance of Herbage Plants. - Characterization of Ice Binding Proteins from Sea Ice Algae. - Isolation and Characterization of Ice Binding Proteins from Higher Plants.