Pseudomonas aeruginosa

This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

CRISPR/Cas9-based genome editing of
Pseudomonas aeruginosa. -
Investigating
Pseudomonas aeruginosa
Gene Function During Pathogenesis using Mobile-CRISPRi. - Engineering green-light-responsive heterologous gene expression in
Pseudomonas. -
Fluorescence-based evaluation of cyclic di-GMP levels in
Pseudomonas aeruginosa. -
Whole-cell biosensors for qualitative and quantitative analysis of quorum sensing signal molecules and the investigation of quorum quenching agents. - A
Pseudomonas aeruginosa
-suitable fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs. - The
Pseudomonas aeruginosa
resistome: permanent and transient antibiotic resistance, an overview. - Biosensors for inducers of transient antibiotic resistance. -
Pseudomonas aeruginosa
soluble pyocins as antibacterial weapons. - Assays for studying
Pseudomonas aeruginosa
secreted proteases. - Single microcolony diffusion analysis in
Pseudomonas aeruginosa
biofilms. - Broad genome sequencing of environmental and clinical strains and genotyping. - Genome-scale analysis of the structure and function of RNA pathways and networks in
Pseudomonas aeruginosa. -
In-depth quantitative proteomics analysis of the
Pseudomonas aeruginosa
secretome. Improving the predictive value of preclinical mouse models of
Pseudomonas aeruginosa
respiratory infection to evaluate antibiotic efficacy. - Emerging
in vitro
models for the study of infection and pathogenesis of
Pseudomonas aeruginosa
and testing of antibacterial agents.