The TNF Superfamily

Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis and osteoclastogenesis.

Assaying NF-kB Activation and Signaling from TNF Receptors. - Dissecting DR3 Signaling. - Modulation of FceRI-Dependent Mast Cell Response by OX40L. - Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK. - Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies. - ß-actin in the Signaling of Transmembrane TNF- -Mediated Cytotoxicity. - Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model. - Analysis of TNF-Mediated Cerebral Pericyte Remodeling. - CD137 in Chronic Graft-Versus-Host Disease. - In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor- and Interleukin-1 in Murine Osteoclastogenesis. - Evasion of TNF- Mediated Apoptosis by Hepatitis C Virus. - TNF- Modulates TLR2-Dependent Responses During Mycobacterial Infection. - Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label. - A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions. - Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys. - Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing. - Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT. - One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra.