This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.
Inhaltsverzeichnis
Isolation and identification of
Yersiniapestis
. - Isolation and characterization of
Yersiniapestis
bacteriophages. - Extraction of chromosomal DNA and plasmid from
Yersinia pestis. -
Extraction of total RNAs from
Yersinia pestis. -
Genotyping of
Yersinia pestis. -
Genome-wide variation analysis of
Yersinia pestis. -
Genome-wide detection of expressed sRNAs in
Y. pestis
with RNA-seq. - Validation of
Y. pestis
sRNAs with nonradiative-labeled Northern Blot. - Determination of transcriptional start sites (TSSs) in Y. pestis with a primer extension assay. - Purification of Hfq-associated RNAs with RNA immunoprecipitation (RIP). - Overexpression of target sRNA in tightly controlled multicopy plasmids. - Target validation of sRNA with a GFP reporter gene fusion system. - Inducible gene expression in
Yersinia pestis. -
Analysis of Yops secretion by
Y. pestis
Type III secretion system. - Co-Immunoprecipitation analysisfor detection of protein-protein interaction in Y. pestis. - Detection of type III secretion system needle assembly in
Yersinia pestis
by cross-linking of YscF. - Evaluation of protein translocation into eukaryotic cells by
Y. pestis. -
Adhesion analysis of
Y. pestis
to the host cells. - Construction of gene deletion mutants in Yersinia pestis. - CRISPR-Cas12a assisted recombineering in Yersinia pestis. - Plasmid curing in
Yersinia pestis. -
Genetic regulation analysis of
Yersinia pestis. -
Investigation of Biofilm formation in
Y. pestis. -
Extraction and purification of F1 capsule antigen from
Y. pestis. -
Screening of regulator responses to
Yersinia pestis
infection with a concatenated tandem array of transcription factor response elements-pull down. - Animal models for
Yersinia pestis. -
Antibiotic resistance evaluation of
Yersinia pestis. -
PCR for detection and identification of
Yersinia pestis. -
Immunological methods for detection and identification of
Yersinia pestis. -
Analysis of fatty acids in
Yersinia pestis
by gas chromatography. - Evaluation of efficacy of
Yersinia pestis
vaccine. - Online resources for
Yersinia pestis. -
Effects of
Y. pestis
on macrophage.
