Plant Tissue Culture Manual

This supplement, containing six chapters, is the first in a series of important works designed to be integrated into the text of the Plant Tissue Culture Manual to maintain it as a valuable source of laboratory methodology

Part 1 Basic techniques - cell and tissue culture of model species: Arabidopsis regeneration and transformation (root explant system); somatic embryogenesis in carrot; low density cultures - microdroplets and single-cell nurse cultures. Part 2 Tissue culture and transformation of crop species: transformation and regeneration of maize protoplasts. Part 3 Propagation and conservation of germplasm: virus elimination and testing. Part 4 Direct gene transfer and protoplast fusion: isolation and uptake of plant nuclei.