A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors.
Inhaltsverzeichnis
Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO. - High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples. - Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis. - In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode. - Measurement of Exhaled Nitric Oxide. - Nitric Oxide Imaging in Neurons Using Confocal Microscopy. - Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry. - A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts. - Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization. - Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase. - Dioxygen-Dependent Metabolism of Nitric Oxide. - Proteomic Method for Identification of Tyrosine-Nitrated Proteins. - Immunohistochemical Detection of S-Nitrosylated Proteins. - Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction. - Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats. - Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase. - Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors. - Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer.
