This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.
Inhaltsverzeichnis
Carbohydrate-based Vaccines: An Overview. - Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations
. -
The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens. - Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure. - Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens. - Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria. - Determination of
N
-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility. -
N-
Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF. - Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines. - Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines. - Identification and Characterization of Carbohydrate-Based Adjuvants. - Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft. - Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods. - Glycoconjugate Vaccines: The Regulatory Framework.
