This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Inhaltsverzeichnis
Chapter 1. General Principles. - Chapter 2. Signals Scattering. - Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction. - Chapter 4. Fluidics. - Chapter 5. Light Sources. - Chapter 6. Optical Benches. - Chapter 7. Detectors and Electronics. - Chapter 8. Signal Analysis. - Chapter 9. The Cytometric File. - Chapter 10. Data Transformation. - Chapter 11. Data Representation. - Chapter 12. Data Analysis. - Chapter 13. Standards, Setup, Calibration, and Control Techniques. - Chapter 14. Fluorochromes Overview. - Chapter 15. Fluorochromes Suitable for Antibody Conjugation. - Chapter 16. Fluorochromes That Bind Nucleic Acids. - Chapter 17. Fluorochromes for the Study of the Cell Features. - Chapter 18. Fluorescent Proteins. - Chapter 19. Spillover and Compensation. - Chapter 20. Artifacts. - Chapter 21. Cell Sorting. - Chapter 22. Non-Conventional Flow Cytometry. - Chapter 23. Statistics: A Cytometric Point of View.
