Euglenozoa

This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics research methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 1 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology.

Chapter 14 is available open access under a Creative Commons Attribution 4. 0 International License via link. springer. com.

Why Euglenozoans. - Isolation and Cultivation of Diplonemids. - Preparation of Diplonemid Samples for Microscopy. - Protocol for the Molecular Identification of Autotrophic Euglenids in Environmental Samples. - Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi. - Poly(A) Profiling for Euglenozoans Using Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena gracilis. - Euglenid Extrachromosomal DNA: Assembly and Annotation. - Nuclear Genome Assembly and Annotation of Kinetoplastids. - Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes. - Cleavage Under Targets and Release Using Nuclease (CUT& RUN) for Trypanosoma brucei Parasites. - Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq. - Transcriptomic Analysis of Trypanosomatids. - Small RNA Profiling in Trypanosomes. - Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) of Metabolites in Heart Samples Infected with Trypanosoma cruzi. - Microbiome Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis. - Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass Spectrometry. - Exploring Protein Interactomes Using TurboID-Directed Proximity Labeling and Mass Spectrometry. - Mapping Trypanosoma Protein Interactome by Proximity-Dependent Biotinylation. - From Genome to Function: Metabolism-Wide Models of Lifecycle Stages of Parasites. - Enhanced Detection of Homology Using Artificial Intelligence in Euglenids.