Mitochondrial Medicine

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional' mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be acomprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow. - Preparation of `Functional Mitochondria A Challenging Business. - Isolation and Quality Control of Functional Mitochondria. - Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient. - Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues. - Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems. - Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes. - Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in
Drosophila
Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging. - Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays. - Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues. - Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria. - Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence. - Assessment of Mitochondrial Ca
²⁺
Uptake. - Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices. - Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis. - Analysis of Mitochondrial Dysfunction during Cell Death. - Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes. - Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane. - Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway. - 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction. - Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology. - High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria. - Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism. - Fluorescence-based Assay for Measuring OMA1 Activity. - Studying Mitochondrial Network Formation by
in vivo
and
in vitro
Reconstitution Assay. - Extraction of Functional Mitochondria Based on the Membrane Stiffness. - A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing. - A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry. -
In vivo
Visualization And Quantification of Mitochondrial Morphology in
C. elegans. -
Assessing Impact of Platinum Complexes on Mitochondrial Functions. -
In-silico
Modeling of the Mitochondrial Pumping Complexes with Markov State Models. - Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a NewMolecular Biosensor.