Plant Biotic Stress

This second volume provides updated and new methods on plant-biotic interactions, including the use of bioinformatics as a tool, as well as various protocols from molecular biology, biochemistry, physiology, pathology, and agricultural sciences. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.

Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols, Volume 2 aims to provide well-established protocols along with recent methodological advancements, enabling accurate, reproducible, and in-depth research, particularly in the context of crop protection.

Comprehensive Collection, Detection, and Identification of Insect Pest-Induced Crop Volatiles for Advanced Pest Management Strategies. - Assessing the impact of weeds on rice growth and productivity: Experimental designs and quantitative approaches. - An Integrated Computational Method for Investigation of Structural and Functional Impacts of Host-Pathogen Interaction. - STA-Fetcher: A Multi-Column Tool for Bulk Retrieval of NCBI Sequences in Plant-Biotic Stress Research. - CRISPR/Cas-mediated genome editing for developing herbicide tolerant rice: A Step-by-step protocol. - An integrated approach to studying the wheat
Diuraphis noxia
interaction for identifying aphid biotypes and assessing plant responses. - Analyses of the cell wall modification of wheat cultivars infested with
Diuraphis noxia. -
Extraction of Amylases from Insects for Testing Inhibitory Compounds from Plants. - Method for Analysing CAZyme Activity from Whole-Body and Saliva Proteome of Russian Wheat Aphid. - In vitro evaluation of effectiveness of antifungal agents against Macrophomina Phaseolina. - Analysis of ß-glucans in the cell walls of plants and fungi using thin-layer chromatography. - Estimation of phenylalanine ammonia-lyase activity for assessing resistance of muskmelon against
Fusarium oxysporum
f. sp.
melonis
infection. - Analysis of Secreted Proteins from
Macrophomina phaseolina
Isolated from Infected
Glycine max
Tissues. - Standardized Workflow for Long Non-coding RNA Prediction and Expression Profiling in Wheat under Stem Rust Stress. - Estimation of DNA methylation in wheat using Reverse Phase Ultra-High-Performance Liquid Chromatography coupled with Mass Spectrometry during leaf rust pathogenesis . - Protocol for collection, identification, and artificial inoculation of
Bipolaris sorokiniana
for functional analyses of wheat spot blotch interactions and germplasm screening. - Tracking Oxidative Bursts in Tomato Pathogen Interactions. - Targeted Gene Expression Modulation Using CRISPR/dCas9 to Investigate Pathogenic Outcomes in Tomato. - Quantification of mitochondrial pyrroline-5-carboxylate in Arabidopsis under
Pseudomonas syringae
infection. - Isolation of AtSWEET Membrane Proteins from Arabidopsis Leaves for host-Pathogen Interaction studies. - Procedure for Grafting Cultivated Eggplant Varieties onto Wild Eggplant Species to Confer Resistance Against
Ralstonia solanacearum. -
Cloning of
Begomoviruses
and
Betasatellites
Associated with
Chilli Leaf Curl Disease.