Bacterial Cell Wall Homeostasis

This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organism's cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.

Atomic
Force Microscopic Analysis of Bacterial Cell Wall Peptidoglycan Architecture. - Ultra-Sensitive
High Throughput Analytical Methods to Identify Novel Components Involved in Cell
Wall Biology and to Study Bacterial Cell Wall Dynamics. - Microarray
Analysis to Monitor Bacterial Cell Wall Homeostasis. - Cell Shaving
and False Positive Control Strategies Coupled to Novel Statistical Tools to Profile
Gram-Positive Bacterial Surface Proteomes. - Differential
Proteomics Based on Multidimensional Protein Identification Technology to Understand
the Biogenesis of Outer Membrane of Escherichia
coli. - Random
Transposon Mutagenesis for Cell-Envelope Resistant to Phage Infection. - Zymographic
Techniques for the Analysis of Bacterial Cell Wall in Bactillus. - Liquid Chromatography-Tandem Mass Spectrometry
to Define Sortase Cleavage Products. - Genetics
and Cell Morphology Analyses of the Actinomyces
oris srtA Mutant. - Construction
of a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope. - Luciferase
Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus. - Extraction
and Analysis of Peptidoglycan Cell Wall Precursors. - Continuous
Fluorescence Assay for Peptidoglycan Glycosyltransferases. - Analysis
of Peptidoglycan Fragment Release. - Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus. - Analysis of Bacterial Cell Surface
Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy. - Biophysical Measurements of Bacterial
Cell Shape. - Coarse-Grained Molecular Dynamics Simulations
of the Bacterial Cell Wall. - Structural
Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.