Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity.
"Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
Inhaltsverzeichnis
Bioinformatics of Antigen-binding Sites. - Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool. - Standardized Sequence and Structure Analysis of Antibody Using IMGT®. - Protein Sequence and Structure Analysis of Antibody Variable Domains. - Generation of Antibody Fragments and Their Derivatives. - scFv by Two-Step Cloning. - Bivalent Diabodies. - Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies). - Miniantibodies. - Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins. - Antibody Cytokine Fusion Proteins with Members of the TNF-Family. - Recombinant Immunotoxins for Treating Cancer. - T Bodies. - Expressing Intrabodies in Mammalian Cells. - Phenotypic Knockdown with Intrabodies. - Disulfide-Stabilized Fv Fragments. - PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics. - Fusion Proteins with Improved PK. - In Vivo Biotinylated scFv Fragments. - Bispecific Diabodies and Single-Chain Diabodies. - Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig ) Molecule. - Isolation of Antigen-Specific Nanobodies. - CDR-FR Peptides. - Production of Antibody Fragments. - Purification and Characterization of His-Tagged Antibody Fragments. - Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium. - Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L. - Purification and Analysis of Strep-tagged Antibody-Fragments. - Production of Antibodies and Antibody Fragments in Escherichia coli. - Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones. - Bioreactor Production of scFv Fragments in Pichia pastoris. - Expression of Antibody Fragments in Transgenic Plants. - Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells. - Recombinant Antibody Molecules in Nanobiotechnology and Proteomics. - Immunoliposomes. - Targeted Polymeric Nanoparticles. -Antibody Microarrays for Expression Analysis. - Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique. - Preclinical and Clinical Development. - Xenograft Mouse Models for Tumour Targeting. - Xenograft Mouse Models for Tumour Targeting. - Imaging Tumor Xenografts Using Radiolabeled Antibodies. - Human Anti-antibody Response. - IP Issues in the Therapeutic Antibody Industry.
