RNA Abundance Analysis

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in
Nicotiana benthamiana
. - Strand-Specific RNA-Seq Applied to Malaria Samples. - Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning. - Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting. - Quantitative Analysis of Plant miRNA Primary Transcripts. - A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq. - Analysis of RBP Regulation and Co-Regulation of mRNA 3 UTR Regions in a Luciferase Reporter System. - Extraction of Small RNAs by Titanium Dioxide Nanofibers. - Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data. - Purification and Analysis of Chloroplast RNAs in
Arabidopsis. -
In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes. - Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs. - Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses. - Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from
Neurospora crassa
and
Fusarium oxysporum
and Analysis of Small RNAs in
Fusarium oxysporum
-Infected Plant Root Tissue. - Studying RNA-Protein Interaction Using Riboproteomics. - Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.