Spermatology

This volume covers a broad range of procedures and protocols that discuss the steps for designing experimental setups for studying sperm, including analytical tools for diverse species. The chapters in this book are organized into 5 parts: Part One looks at the latest classical methods of analysis of sperm quality parameters; Part Two discusses the process of sperm collection in wild and domestic species; Part Three covers spermatozoa, including cryopreservation and analysis of a variety of sperm characteristics; Part Four focuses on molecular determinants in seminal plasma; and Part Five talks about sperm interactions, describing the fine cross-talk of the male gametes with the female genital tract. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Spermatology: Methods and Protocols is a valuable resource for both novel and expert researchers who want to learn more about sperm-related methodologies.

Spermatology: Current Methods to Study Sperm Classical Variables. - Evolutionary Aspects of Sperm Physiology and Its Assessment. - Assessment of Boar Semen Morphology and Morphometry. - Semen Extraction and Analysis in Domestic Animals. - Semen Extraction and Management in Wild Animals: TUMASG Technique. - Semen Extraction and Analysis in Avian Species. - Boar Semen Assessment for the Production of Artificial Insemination Doses. - Semen Evaluation in the Production of Bovine Artificial Insemination Doses. - Sperm Vitrification in Horse and Donkey. - Cryopreservation of Dog Semen. - Rabbit Sperm Cryopreservation. - Cryopreservation of Small Ruminant Sperm and Detection of Sperm Cryocapacitation. - Sperm Cyropreservation in Boars. - Cryopreservation of Horse Sperm. - New Delivery Systems on Sperm Quality Improvement. - Assessment of Sperm Motility with the Use of Computer-Aided Sperm Analysis (CASA). - Bioinformatics on Sperm Subpopulations using Computer Assisted Sperm Analysis (CASA). - Sperm Selection by Colloid Centrifugation. - Bioenergetics of Rodent Spermatozoa. - Flow Cytometry in the Assessment of Sperm Function Parameters: Viability, Acrosomal Integrity, Membrane Stability, and Mitochondrial Status. - Dextran/Swim-Up Procedure for Ram Sperm Selection. - Chromatin Structure Assessment Using Flow Cytometry after DNA Denaturation and Acridine Orange Staining. - Assessment of the Sperm DNA Oxidative Damage by Immunofluorescence and Flow Cytometry. - Sperm Intracellular Calcium Evaluation. - Oxidative Status Measurement in Domestic and Wild Small Ruminants Species. - Evaluation of Oxidative Stress in Mammalian Spermatozoa. - Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm. - Field Emission Scanning Electron Microscopy (FE-SEM) as an Approach for Membrane Surface Mapping in Cell Biology. - Detection of Sperm Membrane Proteins by Immunocytochemistry, Scanning and Transmission Electron Microscopy, and Western Blotting. - Detection and Quantification of mRNA in Mammalian Spermatozoa. - Techniques to Determine Mammalian Sperm Capacitation. - Disulfide Bridges Assessment in the Sperm Chromatin by Flow Cytometry. - Analysis of the Chromatin Structure by Chromomycin A3 (CMA3) and Flow Cytometry. - Isolation of High-Quality RNA from Mammalian Spermatozoa for Transcriptome Studies. - Prediction of CircRNAs in Mammalian Spermatozoa from RNA Sequencing Data. - Reduced Representation Bisulfite Sequencing Library Preparation for Sperm DNA Methylation Analyses: Manuel and Automated Methods. - Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature. - High Throughput Analysis of Post-Meiotic Male Germ Cells Using Image Flow Cytometry. - Assessment of Bacterial Contamination in Semen. - A Size-Exclusion Chromatography-Based Procedure for Isolating Extracellular Vesicle Subsets from Porcine Semina Plasma. - Molecular Determinants in Pig Semen: Verified Protocols for Cytokine Detection and Quantification. - Molecular Determinants in Semina Plasma and Spermatozoa: Non-Targeted Metabolomics. - Seminal Plasma Proteomics using Filter Aided Sample Preparation. - Assessing Seminal Fluid Interaction with Female Reproductive Tract Tissues: In Vivo and In Vitro Models for Mouse. - Seminal Fluid Interaction with Female Reproductive Tract Epithelial Cells: In Vitro Models for Human. - Impact of Seminal Plasma on the Embryonic Development and Uterine Environment in Pigs. - Assessment of Sperm Binding Capacity in the Reservoir using an In Vitro Model of Oviduct Epithelial Spheroids in Cattle. - Characterization of Ovine Cervical Mucus and Implementation of a Sperm-Mucus Penetration Test Using Fluorescently Labeled Sperm.